Extended Data Fig. 3: Legumain consistently exhibits reduced cysteine reactivity in H. pylori-infected cells under various infection conditions.
From: An infection-induced oxidation site regulates legumain processing and tumor growth
(a) Western blot analysis of legumain in H. pylori-infected (H. pylori G27MA, MOI 50, 18 h) and uninfected KATO III cells before (input) and after IA enrichment. (b) Western blot analysis of legumain in uninfected AGS cells and AGS cells infected with the H. pylori strains G27 or PMSS1 (MOI 50, 18 h) before (input) and after IA enrichment. (c) Western blot analysis of legumain in uninfected AGS cells and AGS cells infected with H. pylori G27MA for 18 h at MOI 25 or 50 before (input) and after IA enrichment. (d) Quantification of AGS cell viability following infection with H. pylori G27MA (MOI 25, 18 h) or incubation with media alone for 18 h. Data represent three independent experiments. Bars represent means ± SD. Each circle represents an independent experiment. n.s., not significant, by two-tailed unpaired t-test. Western blot analyses were performed two (c) or three (a, b) times with consistent results. Band intensities of mature legumain and ACAT1 were normalized by the corresponding band intensities in the uninfected sample.
