Extended Data Fig. 8: SETD2 depletion halts lymphoma growth.
From: Drug addiction unveils a repressive methylation ceiling in EZH2-mutant lymphoma
a, Immunoblot showing H3K27me3 and H3K36me3 levels in lymphoma cell lines treated with 5 μM SETD2-IN-1 for 72 h. Short exposure (SE); long exposure (LE). b, Bar plots showing SETD2 expression at the transcript level after SETD2 knockdown for 72 h. c, Bar plots showing cell proliferation after SETD2 knockdown for 10 days. d, Immunoblot showing γH2AX and H3K36me3 levels in Karpas-422 treated with 5 μM SETD2-IN-1 for 10 days or 10 μM etoposide for 24 h. Results in a, d are representative of two independent experiments. Data in b-c are are represented by mean ± s.e.m of n = 3, except for b. Karpas-422 and SUDHL4 shSETD2 #1 n = 5.
