Fig. 4: MIA biosynthetic genes are partitioned into three discrete cell types in the C. roseus leaf.
From: Single-cell multi-omics in the medicinal plant Catharanthus roseus
a, UMAP of gene expression in C. roseus leaves (n = 15,437 cells). b, Gene expression heatmap of the MIA biosynthetic pathway for bulk and single-cell transcriptomes. Genes are arranged from upstream to downstream. Previously reported cell-type-specific expression18,19,20,21,22 are confirmed and marked with asterisks. For the single-cell gene expression heatmap, color scale shows the average scaled expression of each gene at each cell type (Methods). Dot sizes indicate the fraction of cells where a given gene is expressed at a given cell type. c, Gene coexpression network for MIA biosynthetic genes using leaf scRNA-seq data. Each node is a gene. Larger size nodes represent previously characterized genes. Edges represent coexpression (FDR < 0.01; Methods). See Supplementary Fig. 1 and Supplementary Table 6 for a list of gene name abbreviations. There are 38 biosynthetic genes and two transporters, among which SLTr and STRTr are transporters. See Supplementary Table 9 for the membership of genes in each module. E, epidermis; I, idioblast; M, mesophyll; V, vasculature; Un, unassigned.
