Fig. 6: Reduction of an iminium to form anhydrovinblastine.
From: Single-cell multi-omics in the medicinal plant Catharanthus roseus
Discovery of ADH20, and comparison of kinetic parameters against THAS2 and other ADHs. a, A short chemical scheme showing coupling and reduction. b, Expression heatmap at single-cell type resolution. Color scale shows the average scaled expression of each gene at each cell type (Methods). Dot sizes indicate the fraction of cells where a given gene is expressed at a given cell type. c, Specific activity of the five idioblast-localized ADH enzymes recombinantly expressed in E. coli and tested in vitro for activity toward the AHVB iminium. n = 3 for all assayed enzymes. d, Heatmap showing cells as columns and compounds as rows from the scMet experiment (n = 586 cells).
