Extended Data Fig. 10: Metabolomic and immunoblotting analysis verified the ability of pyruvate in entering HCT116 cells and rescuing TSA-induced apoptosis.
From: Chemoproteomic mapping of the glycolytic targetome in cancer cells
(a) EICs of intracellular pyruvate in HCT116 cells without and with pyruvate administration for 24 hr (n=3 biologically independent samples). (b) Relative ion abundance of intracellular pyruvate using data in (a). Data represent the mean ± SEM (n=3 biological samples) and P value was determined using an unpaired two-tailed Student’s t-test. (c) Gating strategy to sort TSA-induced apoptotic HCT116 cells without and with pyruvate pretreatment as specified in Fig. 6g. (d) Representative immunoblots of the apoptotic protein markers, including cleaved PARP and cleaved caspase-9, in HCT116 cells treated as specified in Fig. 6g. β-Tubulin was used as the loading control. Statistical analyses of the band intensities of the protein markers are shown in the bottom panel, where data represent the mean ± SEM (n=3 independent experiments) and P values were determined using ordinary one-way ANOVA with Tukey’s multiple comparisons test.
