Extended Data Fig. 3: TRAP complements thermal stability-based target discovery approaches.
From: Chemoproteomic mapping of the glycolytic targetome in cancer cells
(a) Analysis of protein melting behaviors using the human HEK293T cell meltome data (PXD011929) identified five clusters as shown here and in Fig. 2a. (b) Nonmelters displaying resistance to thermal denaturation were identified in the meltome data of HaCaT, HepG2 and colon cancer spheroids cells (PXD011929). (c) GO BP analysis of the nonmelters shown in (b) using a modified Fisher’s Exact test from DAVID bioinformatics website. (d) Dose-responsive TRAP detected dose-dependent increased abundance of the TRPtype C of PSMB1-K164, implying dose-dependent decreased accessibility at K164 following bortezomib incubation. The experiment was conducted once. (e) Dose-responsive TRAP curves for bortezomib’s target candidates that were assigned by single-dose TRAP experiment shown in Fig. 2f (the bona fide target PSMB1 excepted). The experiment was conducted once.
