Extended Data Fig. 1: Characterization of Fubi-based probes.
From: Molecular basis for ubiquitin/Fubi cross-reactivity in USP16 and USP36
a. Schematic of the chemoproteomics experimental workflow used to identify Fubi proteases. b. Characterization of indicated samples by intact protein mass spectrometry. HA-Fubi-MesNa and derived proteins featured Cys57 mutations to suppress reaction of the thiol with the VS warhead during probe assembly. Analogously mutated and wild-type HA-Fubi-PA probes were prepared for comparison. *, species with a C-terminal carboxylate which was separated during purification. c. Overview of different versions of HA-tagged Fubi probes used in this work.
