Extended Data Fig. 2: Reactivity of USP DUBs in HEK293 lysate.
From: Molecular basis for ubiquitin/Fubi cross-reactivity in USP16 and USP36
a. Assessment of endogenous USP16 and USP36 reactivity towards Ubiquitin and Fubi probes. HEK293 lysate was incubated with indicated probes (1 h, 37 °C) and reactivity was assessed by Western blot. Uncropped blots are provided as Source Data. b. Cellular labeling experiment as in a with indicated HA-Fubi-PA probes, demonstrating that mutation of Cys57 in Fubi does not affect reactivity toward USP36. c. Assessment of additional USP probe reactivity. Flag-tagged, full-length USP7, USP10 and USP48 were overexpressed in HEK293 and analyzed as in Fig. 1d. d. Depiction of cellular roles of USP16 and USP36. Processes to which USP16 and USP36 activity has been connected are listed, with substrates in parentheses. e. Assessment of endogenous Fubi-S30 (FAU) accumulation upon USP36 and USP16 depletion in MCF7 cells after 48 h. Compare to Fig. 1g for HEK293 cells.
