Extended Data Fig. 4: The NuRD complex is required for ENO1/YAP1-mediated HPGD gene repression.
From: ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism
a, b, qPCR (a) and immunoblotting (b) analysis of HPGD expression levels in PLC cells treated with 1 μM TSA, or expressing shRNAs targeting MTA2, HDAC1, or HDAC2 (mRNA data: n = 3 independent assays). Actin served as a negative control. c, PLC cells expressing Flag-tagged YAP1 or ENO1 were further infected with shRNA viruses targeting HDAC1. Protein levels of YAP1, ENO1, Flag, HDAC1, and HPGD were measured by immunoblotting analysis. Actin served as a negative control. d, A schematic representation of the ENO1-mediated activation of YAP1 transcriptional regulation of the PLCB1 and HPGD loci. Illustration created with Biorender.com. Immunoblots are representative of three independent experiments. Error bars denote the mean ± s.e.m. (a). Statistical analyses were performed by two-tailed Student’s t-test (a).
