Extended Data Fig. 8: COX2 is responsible for ENO1/YAP1-mediated tumor growth.
From: ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism
a, b, Cell growth was measured in ENO1- or YAP1-overexpressing PLC cells (a) or Hep3B cells (b) infected with shRNA viruses targeting COX2 (n = 3 independent assays). Protein levels of ENO1, YAP1, and COX2 were measured by immunoblotting analysis. Actin served as a loading control. c-f, Equal numbers of Hep3B cells expressing ENO or YAP1 that further knockdown COX2 were injected subcutaneously into the flanks of BALB/c nude mice (n = 5 mice in each group). Tumor sizes were measured every 3 days using calipers (c, d). Tumor weights (e, f) were determined at the end of the experiment (day 29). g, h, Protein levels of ENO1, YAP1, and COX2 in tumor lysates of Extended Data Fig. 8e, f were measured by immunoblotting analysis. Actin served as a loading control. Immunoblots are representative of three independent experiments (a,b). Error bars denote the mean ± s.d. (a,b) or mean ± s.e.m. (c-f). Statistical analyses were performed by two-tailed Student’s t-test (e,f), or two-way ANOVA with tukey’s multiple comparisons test (a-d).
