Extended Data Fig. 1: ENO1 regulates the expression of YAP1 target genes.
From: ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism
a, Protein copy numbers of glucose metabolism enzymes in NIH3T3 mouse fibroblast cells (Schwanhausser et al., 2011), and HeLa cells (Hein et al., 2015; Itzhak et al., 2016). b, Venn diagram showing the 21 overlapped target genes enriched by ENO1 IP in our RIP-seq data from HEK293T-Flag-ENO1 cells and the previously published HITS-GBP-CLIP data from HeLa-ENO1-YFP cells (Castello et al., 2012). c, qPCR-based expression analysis of known YAP1 target genes including NPPB, CYR61, CTGF, S1PR1, NEXN, NTF3, KDR, CPA3, PSG1, CCDC80, EGR3, EGR4, DDIT4, and TRAIL in PLC cells expression shRNAs targeting ENO1 or YAP1 (n = 3 independent assays). Error bars denote the mean ± s.e.m. (c).
