Extended Data Fig. 6: Flow cytometry of WT-∆edd co-cultures.
From: A parallel glycolysis provides a selective advantage through rapid growth acceleration
At least 10,000 events were recorded per sample. Singlets were gated based on FSC-A (forward scattered area) and FSC-H (forward scatted height) intensities in a way to minimize the frequency of aggregates observed. Non-fluorescent (WT), GFP expressing (WT + GFP) and mCherry expressing (∆edd+mCherry) cells were used as controls and analyzed on the day of experiments to determine the compensation of spectral overlap between fluorophores and gating quadrants for the glucose upshift (top) and ammonia upshift (bottom) experiments. Samples at the beginning (left) and end (right) of co-culture intermittent nutrient supply experiments are shown as examples.
