Extended Data Fig. 7: Mutations in the LTEE reflect selective pressure.
From: A parallel glycolysis provides a selective advantage through rapid growth acceleration
a,b, Mutation frequencies in genes deviated from the Poisson distribution. Genes with the same number of mutations were counted, and the normalized counts were fit to a Poisson distribution. Poor fitting for genes with 2 or more mutations suggested a driving force toward improved fitness. Error bars represent the standard deviation (n = 212 all possible sets of twelve independent clones). c,d, The total number of mutations in individual genes across all 24 strains at the 50,000th generation. For each gene, we counted the number of mutations in all 24 strains. A few genes were consistently and heavily mutated: malT is a regulator for the maltose regulon that is also involved in λ phage infection; yeeJ is an autotransporter protein; metH is the gene for methionine synthase; pykF is pyruvate kinase, the last step of glycolysis; thrA is a bifunctional aspartokinase and homoserine dehydrogenase; nadR is a regulator for NAD biosynthesis; hsIU is a putative ATP dependent protease; and iclR is a regulator for isocitrate lyase of the glyoxylate cycle.
