Extended Data Fig. 9: Confocal imaging, western blot, and sample traces of WT and selected mutants transiently expressed in HEK293 cells.
From: Structure and mechanism of a neuropeptide-activated channel in the ENaC/DEG superfamily
a, Confocal imaging. From left to right, three images in each box represent the indicated AcFaNaC variant fused with GFP, membrane marker KRas GTPase fused with mCherry, and merge, respectively. The scale bars represent 5 μm. The experiment was repeated twice independently with similar results. b, Western blotting. T stands for total extract after detergent solubilization, whereas S stands for supernatant after centrifugation. The experiment was repeated twice independently with similar results. c, Sample traces. FMRFamide (30 μM) was applied for each measurement as indicated by bars.
