Extended Data Fig. 3: Screens of an NNK-mutagenized PYR1*MANDI library yield receptors for diverse organophosphate ligands.
From: An orthogonalized PYR1-based CID module with reprogrammable ligand-binding specificity
Characterization of organophosphate responsive sensors isolated by screening a library of NNK-mutagenized PYR1*MANDI variants. The mutations in each sensor isolated are shown in the table, and the parental PYR1*MANDI sequences are shown at the top. Y2H assays using different ligand concentrations are shown on the right. The mutants characterized were isolated in growth-based Y2H screens and showed reproducible ligand-stimulated growth; however, a small number of the clones show marginal β-galactosidase activity in the assays. This table includes these because the initial growth on 100 µM ligand was reproducible.
