Extended Data Fig. 2: Cryo-EM images of apo-GPR101-Gs complex and data processing.
From: Structure of GPR101–Gs enables identification of ligands with rejuvenating potential
a-b, Representative Cryo-EM micrograph (Scale bar: 50 nm) (a) and 2D class averages (b) of the GPR101-Gs complex. Representative Cryo-EM micrographs from 6,161 movies and representative 2D class averages determined using approximately 1.82 million particles from 2D classification were shown. c, Flow chart of cryo-EM data processing of GPR101-Gs complex leading to the construction of electron density maps with an overall resolution of 2.89 Å. d, 3D density map of the GPR101-Gs complex colored according to local resolution. e, Angular distribution of the particles used for the final reconstruction of the GPR101-Gs complex. f, Gold-standard Fourier shell correlation (FSC) curves for GPR101-Gs complex.
