Extended Data Fig. 8: Tethering of ER and mitochondria contributes to ferroptosis.
From: CGI1746 targets σ1R to modulate ferroptosis through mitochondria-associated membranes
a-b. NCI-H1299 cells were transfected with two individual siRNAs specially against MFN2 for 48 hours, then treated with increased concentrations of RSL3 (a) or erastin (b) to evaluate cell death. c. NCI-H1299 cells were transfected with siRNA specially against MFN2 for 48 hours, then subjected to 1 μM RSL3 for 8 hours to detect lipid peroxidation by C11-BODIPY. d-e. NCI-H1299 cells were transfected with two individual siRNAs specially against MFN1 for 48 hours, then treated with increased concentrations of RSL3 (d) or erastin (e) to evaluate cell death. f. NCI-H1299 cells were transfected with siRNA specially against MFN1 for 48 hours, then subjected to 1 μM RSL3 for 8 hours to detect lipid peroxidation by C11-BODIPY. g-h. NCI-H1299 cells were transfected with siRNA specially against PACS2 for 48 hours, then subjected to different concentrations of RSL3 (g) or erastin (h) treatments to measure cell viability. i-j. NCI-H1299 cells were transfected with siRNA specially against VAPB for 48 hours, then subjected to different concentrations of RSL3 (i) or erastin (j) treatments to measure cell viability. Viability data represent mean ± s.d. of n = 3 replicates from one representative of three independent experiments. Flow cytometry data show one representative out of three replicates from one representative of three independent experiments.
