Extended Data Fig. 9: Structure determination of human KCNQ2-Ebio1-S1 complex.
From: A small-molecule activation mechanism that directly opens the KCNQ2 channel
a, Size-exclusion chromatography of KCNQ2-CaM on Superose 6 and SDS-PAGE analysis of the final sample. n = 3 independent experiments. b, Representative cryo-EM micrograph of KCNQ2-Ebio1-S1. n = 3 independent experiments. c, Representative 2D classes. d, Flowchart of image processing for KCNQ2-Ebio1-S1 particles. e, The density map of KCNQ2-Ebio1-S1 is colored by local resolution. The local resolution is estimated with RELION 3.0 and generated in Chimera. f, EM densities for Ebio1-S1 in the KCNQ2 channel. Gray meshes represent EM densities for Ebio1-S1 and its surrounding residues. g, The Gold-standard FSC curves of the final 3D reconstruction of KCNQ2-Ebio1-S1, and the FSC curve for cross-validation between the map and the model of KCNQ2-Ebio1-S1. h, The weak and isolated densities are observed between VSD and PD in the map of KCNQ2-Ebio1-S1 complex. The EM densities are contoured at the level of 0.01 in UCSF ChimeraX.
