Extended Data Fig. 7: OligoD insertion into HA-head of H2 HA.
a, Location of the oligoD insertion site (S156) on the head of H2 HA. b, Size-exclusion purification and gel electrophoresis of H2 HA and reoH2HA. Gels were stained with Coomassie brilliant blue. c, SEC-MALS analysis of H2 HA and reoH2HA on a Superdex 200 column. UV and LS indicate absorbance at 280 nm and light scattering signals, respectively. d, Thermal melting profiles and Tm of H2 HA and reoH2HA. Data are presented as mean ± s.d. in the dot plots (n = 3 samples per group). e, Epitopes targeted by head-directed (8F8 and 8M2) and stem-directed mAbs (MEDI8852 and FI6v3) on H2 HA. Red spheres indicated oligoD insertion sites (S156). f, Binding of head-directed (8F8 and 8M2) or stem-directed mAbs (MEDI8852 and FI6v3) to H2 HA or H2 HA-12D (H2 HA with 12D inserted at its C-terminus) on streptavidin-coated (left) or alum-coated (right) ELISA plates. Data are presented as mean ± s.d. (n = 4 technical replicates). g, Immunogold labeling (left, scale bar, 1 cm) and TEM imaging (right, scale bar, 100 nm) of alum complexed with H2 HA or reoH2HA.
