Extended Data Fig. 8: Immunization of oligoD-modified HA.
a, A prime-boost immunization study with H7 HA-12D adjuvanted with alum alone or alum/CpG via subcutaneous injection in BALB/c mice (n = 5 mice per group). b, Serum H7 HA-specific IgG titers over time. Antibody titers of weeks 3 and 7 from the two groups are plotted on the right for comparison. c, Cross-reactive binding of different group 1 (H1 NC/99, H1 CA/09, H2 JP/57, H5 VT/04) and group 2 HAs (H3 VC/75, H7 NT/27, H7 SH/13) by week-7 antisera (n = 10 mice per group from the immunization study in Fig. 5e). d, Serum H2 HA-specific IgG titers over time (n = 10 mice per group from the immunization study in Fig. 6a). e, Cross-reactive binding of different group 1 (H1 NC/99, H1 CA/09, H2 JP/57, H5 VT/04) and group 2 HAs (H3 VC/75, H7 NT/27, H7 SH/13) by week-12 antisera (n = 10 mice per group from the immunization study in Fig. 6a). f,g, IgG subtypes of week-12 sera raised from low- (f) or high-dose (g) CpG coupled with alum. Dashed lines indicate the limit of quantification in b-g. IgG titers are presented as the geometric mean of the log10-transformed values in b-g. Comparison of two groups was performed using the two-tailed Mann–Whitney U-test in b,c,e. P values of 0.05 or less were considered significant.
