Extended Data Fig. 4: 3D fluorescence assay of free NBSI and Clivia-bound NBSI.
From: Structural basis of a small monomeric Clivia fluorogenic RNA with a large Stokes shift
(a) 3D fluorescence spectrometry of NBSI in water, the maximum excitation and emission wavelengths are 563 nm and 603 nm, respectively. (b) 3D fluorescence spectrometry of NBSI in buffer. The maximum excitation and emission wavelengths are 562 nm and 602 nm, respectively. (c) 3D fluorescence spectrometry of NBSI-Clivia complex in buffer. The maximum excitation and emission wavelengths are 529 nm and 584 nm, respectively. In 3D fluorescence assay, the initial difference ‘Delta start’ between emission wavelength and excitation wavelength was 10 nm, the increment of wavelength difference ‘Delta increment’ was 5 nm, and the maximum wavelength difference ‘Delta stop’ was 190 nm.
