Extended Data Fig. 5: 3D fluorescence assay of fluorophore NBSI565 and NBSI618.
From: Structural basis of a small monomeric Clivia fluorogenic RNA with a large Stokes shift
(a) Chemical structure of NBSI571, NBSI565 and NBSI618. (b) The photophysical parameters of NBSI and derivatives, the data was cited for our previous research (Nature Methods 20(10): 1563-1572). (c) 3D fluorescence assay of fluorophore NBSI565 in the presence of buffer (green) or Clivia (red). In the presence of buffer, Free NBSI565 exhibits two excitation and emission wavelengths peaks at approximately (382 nm, 582 nM) and (552 nm, 591 nm), while Clivia-bound NBSI565 exhibits a single excitation and emission wavelengths peak at (491 nm, 566 nm). (d) 3D fluorescence assay of fluorophore NBSI618 in the presence of buffer (green) or Clivia (red). In the presence of buffer, Free NBSI618 exhibits two excitation and emission wavelengths peaks at approximately (423 nm, 623 nm) and (577 nm, 642 nm), while Clivia-bound NBSI618 exhibits a single excitation peak at (518 nm, 623 nm).
