Fig. 3: Fluorescence assay of the Clivia aptamer and mutants.
From: Structural basis of a small monomeric Clivia fluorogenic RNA with a large Stokes shift
a,b, Fluorescence assay of the Clivia aptamer (a) and structure-guided mutants (b). Here, Del-2bp-P1 indicates that G2-A3 and U34-C35 were removed from stem P1, Ad-2bp-P1 indicates that G(−1)-G0 and C37-C38 were added to stem P1, Del-2bp-P2 indicates that U16-G17 and C26-A27 were removed from stem P2 and Ad-2bp-P2 indicates that C-C and G-G were added to the apical end of stem P2. The activated fluorescence of NBSI by Clivia mutants from three independently repeated experiments is normalized for comparison with the WT Clivia aptamer. Data represent the mean ± s.d. from three replicates. c, The 3D fluorescence assay of fluorophore NBSI in the presence of H2O (blue), buffer (green) or Clivia (red). In the presence of water or buffer, free NBSI exhibits two excitation and emission wavelength peaks at approximately (460 nm, 600 nm) and (560 nm, 600 nm), while Clivia-bound NBSI exhibits single excitation (529 nm) and emission (584 nm) wavelength peaks.
