Extended Data Fig. 4: Expression profiles of shortlisted candidate genes.
Candidates were filtered by PCC (>0.885) to SobAS1, annotation with one of InterPro domains of biosynthetic interest (IPR001128 cytochrome P450; IPR002213 UDP-dependent glycosyltransferase; IPR003480 and IPR001563 acyltransferases) and absolute read count (>1000) in the flower. SoSDR1 and SoGH1 has also been included. The heatmap shows library normalized log2 read counts scaled by row (gene) and was constructed using Heatmap379. Gene ID, annotation, PCC to SobAS1 and absolute read count in flower organ (mean, n = 4) are also listed for each candidate. Genes shown in bold are functional saponarioside biosynthetic genes identified and characterized in this study. Full expression data are available as Source Data.
