Extended Data Fig. 4: MM-JH-1 is not labeling lipids, RNAs, or GPI-anchors in HeLa cells.
From: Selective bioorthogonal probe for N-glycan hybrid structures
(a) Neither nonionic detergent Triton-X100, nor organic solvent acetone has any effect on the MM-JH-1 labeling. All quantification of images was done by normalizing mean fluorescent signal to DAPI (blue). N = 3 independent biological replicates; n = 25 individual cells chosen for quantification of the confocal images. An ordinary one-way ANOVA test was performed, P values are shown, and all error bars represent standard deviation with mean as center. Quantification is shown to the below right corner of the images. (b) RNAse treatment has no effect on MM-JH-1. All quantification of images was done by normalizing mean fluorescent signal to DAPI (blue). N = 3 independent biological replicates; n = 25 individual cells chosen for quantification of the confocal images. A two-sided unpaired t-test was performed, P values are shown, and error bars represent standard deviation with mean as center. Quantification is shown to the below of the images. (c) MM-JH-1 labeling in HeLa cells remained unchanged after phospholipase C (PLC) treatment. N = 4 independent biological replicates. A two-sided unpaired t-test was performed, P values are shown, and error bars represent standard deviation with mean as center. Quantification is shown to the below of the images.
