Extended Data Fig. 5: Glucosidase inhibitor 1-deoxynojirimycin (DNJM) reduced MM-JH-1 labeling.
From: Selective bioorthogonal probe for N-glycan hybrid structures
Increasing concentration of glucosidase inhibitor 1-deoxynojirimycin (DNJM) reduced MM-JH-1 labeling (green, detected with AF 488 staining on confocal images). Augmented signals of ConA (red, detected with ConA-Texas red staining on confocal images. All quantification of images was done by normalizing mean fluorescent signal to DAPI (blue). N = 3 independent biological replicates; n = 25 individual cells chosen for quantification of the confocal images. Scale bar = 50 mm and 10 mm for zoomed in images. An ordinary one-way ANOVA test was performed, P values are shown, and all error bars represent standard deviation with mean as center. Quantifications are shown to the right of the images.
