Extended Data Fig. 9: Change in colocalization between fibrillarin signal and MM-JH-1 with Actinomycin D treatment.
From: Selective bioorthogonal probe for N-glycan hybrid structures
Actinomycin D treatment was used to disrupt the nucleolus. Colocalization of fibrillarin (red, detected with anti-nucleolar marker fibrillarin antibody) with MM-JH-1 (green, detected with AF 488 signal on confocal images) was examined. Colocalization was determined by Pearson’s R value as indicated to the right of the image. N = 3 independent biological replicates, n = 10 individual cells chosen for quantification of the confocal images. Error bars represent standard deviation with mean as center. Scale bar = 50 mm and 10 mm for zoomed in images.
