Extended Data Fig. 1: Assessment of concentration dependent labeling of MM-JH-1 in HeLa cells.
From: Selective bioorthogonal probe for N-glycan hybrid structures
HeLa Cells incubated with different concentrations (25 μM-500 μM) of MM-JH-1 were collected to check the extent of cellular uptake and the effect on O-GlcNAcylation. (a) HeLa cells collected by physical scraping. N = 4 independent biological replicates. (b) HeLa cells collected by trypsinization. N = 4 independent biological replicates. An ordinary one-way ANOVA test was performed, P values are shown, and all error bars represent standard deviation with mean as center. Quantifications are shown to the right of the images.
