Extended Data Fig. 8: Confocal immunofluorescence microscopy of endogenous FUT10 protein.
From: FUT10 and FUT11 are protein O-fucosyltransferases that modify protein EMI domains
HeLa cells (a) or SCTI003-A human induced pluripotent stem cells (b) were stained for either the ER-marker protein disulfide isomerase (PDI) (red), or the cis-Golgi marker (GM130), alongside FUT10 (green) and DNA (DAPI, grey). Stained cells were imaged by confocal microscopy and a single slice is shown (n = 4). Scale bar is 10 μm. c, Confocal immunofluorescence microscopy images (n = 2) obtained from the Human Protein Atlas67 of human U2OS osteosarcoma cells stained for ether the ER-marker calreticulin (CALR) (red), endogenous FUT10 protein (green) and DNA (DAPI, blue).
