Extended Data Fig. 6: Structure determination of KCNQ2-Ebio3 complex.
From: Small molecule inhibits KCNQ channels with a non-blocking mechanism
a, Flowchart of image processing for KCNQ2-Ebio3 particles, and representative cryo-EM micrograph of KCNQ2-Ebio3 (inset). n = 3 independent experiments. b, Size-exclusion chromatography of KCNQ2-CaM on Superose 6 and SDS-PAGE analysis of the final sample. n = 3 independent experiments. c, The gold-standard FSC curves of the final 3D reconstruction of KCNQ2-Ebio3, and the FSC curve for cross-validation between the map and the model of KCNQ2-Ebio3. d, The angular distribution of particles that gave rise to the KCNQ2-Ebio3 cryo-EM map reconstruction. e, The density map of KCNQ2-Ebio3 is colored by local resolution. The local resolution is estimated with RELION 3.1 and generated in Chimera.
