Extended Data Fig. 2: The effects of Ebio2 on KCNQ2.
From: Small molecule inhibits KCNQ channels with a non-blocking mechanism
a, Current-voltage relationship of the KCNQ2 channel in response to 10 nM Ebio2. b, Normalized activation (left) and deactivation (right) phase from full traces in the absence and presence of 10 nM Ebio2. The test voltages were +50 mV and –120 mV, respectively. c, Analysis of activation (left) and deactivation (right) time constants for the KCNQ2 channel in the absence and presence of 10 nM Ebio2. n = 11 biological replicates. d, Representative single-channel recordings from inside-out patches of KCNQ2 at +50 mV in the absence and presence of 1 μM Ebio2 (left), and corresponding all-point amplitude histograms fitted by Gaussian distributions (red solid line, right). e,f, Histogram showing the single-channel conductance (e) and the open probability (f) of KCNQ2 in the absence and presence of 1 μM Ebio2. n = 5 biological replicates. g, Concentration-dependent curves of Ebio2 on KCNQ members, obtained from the +50 mV traces at steady state. h, Concentration-dependent curves of Ebio2 effects on the half-activation voltage shift (ΔV1/2) for KCNQ members. The holding potential was −80 mV. The KCNQ current was elicited by a series of voltage stepping from −90 mV to +60 mV in 10 mV increments (a,g,h). The error bars represent the mean ± s.e.m. of each data point calculated from 3–14 biological replicates. Statistical analysis was performed using an unpaired two-tailed Student’s t-test.
