Extended Data Fig. 3: The effects of Ebio3 on KCNQ2.
From: Small molecule inhibits KCNQ channels with a non-blocking mechanism
a, Current-voltage relationship of the KCNQ2 channel in response to 10 nM Ebio3. The holding potential was −80 mV. The KCNQ2 current was elicited by a series of voltage stepping from −90 mV to +60 mV in 10 mV increments. b, The normalized activation (left) and deactivation (right) phase from full traces in the absence and presence of 10 nM Ebio3. c, Analysis of activation (left) and deactivation (right) time constants for the KCNQ2 channel in the absence and presence of 10 nM Ebio3. n = 5 biological replicates. d, Representative current traces of KCNQ2 obtained from whole-cell patch-clamp recordings before (black) and after (blue) the application of 1 nM Ebio3. The holding potential was −80 mV. The KCNQ2 current was elicited by a 2,000-ms voltage step to +50 mV. e, Effect of 1 nM Ebio3 on the extent of inactivation assessed using a three-pulse (P1–P3) protocol with a holding voltage of −80 mV. P1 was to +50 mV, P2 was from −90 to +50 mV in 20 mV increments for 1,500 ms, and P3 was to +50 mV to assess the fraction of inactivated channels (insert). f, Fraction of non-inactivated channels during each P2 voltage step before (black) and after (blue) the application of 1 nM Ebio3, obtained by measuring the peak current at P3 normalized to P1. The error bars represent the mean ± s.e.m. n = 10 biological replicates. Statistical analysis was performed using an unpaired two-tailed Student’s t-test.
