Extended Data Fig. 5: Acetylomes of young and aged HSCs and MPPs.
From: Acetylation profiling by Iseq-Kac reveals insights into HSC aging and lineage decision
a, Chord diagram showing the classes of acetylated proteins identified in HSCs and MPPs. b-e, Proteins were categorized into five intervals (R1 to R5) based on average protein abundance ranking, and the proportion of acetylated proteins in each interval was calculated and compared. b, Comparison of protein classes in lowest-abundance proteins intervals (R5), based on our proteome data and Zaro et al.’s proteome data. c, Distribution of acetylated peptides identified by Iseq-Kac across five intervals (R1 to R5), based on protein intensity ranking from our study and Zaro et al.’s study. d,e, Distribution of acetylated peptides identified by classic affinity enrichment-based method across five intervals based on protein intensity ranking. Proteome and acetylome data were obtained from Krug et al.’s study (d) and Yongchao et al.’s study (e). f, Dot blotting assays to confirm the specificities of ALDH2-K430ac and ZNF207-K398ac antibodies using peptides containing acetyllysine (Modified) or unmodified lysine (Unmodified). Each dot contained 10 ng, 100 ng, and 1 µg of peptides, respectively. Representative blots from n = 2 independent experiments.
