Extended Data Fig. 3: Evaluation of proteome and acetylome data of HSCs and MPPs.

a, Gating strategy for FACS sorting or analysis of Lin^- (CD3e^-Ter119^-B220^-Gr1^-CD11b^-), HSC (Lin^-CD127^-Sca1⁺c-Kit⁺CD150⁺CD48^-CD34^-), CD150^hi HSC, CD150^lo HSC, MPP1 (Lin^-CD127^-Sca1⁺c-Kit⁺CD150⁺CD48⁺), MPP2 (Lin^-CD127^-Sca1⁺c-Kit⁺CD150^-CD48⁺CD135^-) and MPP3 (Lin^-CD127^-Sca1⁺c-Kit⁺CD150^-CD48⁺CD135⁺), GMP (Lin^-CD127^-Sca1^-c-Kit⁺CD34⁺FcγRIII/II⁺), CMP (Lin^-CD127^-Sca1^-c-Kit⁺CD34⁺FcγRIII/II^-), CLP (Lin^-CD127⁺) and MEP (Lin^-CD127^-Sca1^-c-Kit⁺CD34^-FcγRIII/II^-). b-c, Representative data of one of twelve to eighteen independent experiments, for young (b) and aged (c) HSCs and MPPs. d, Flowchart of generating cell-specific Kac libraries for HSCs and MPPs. e, Venn diagrams depicting the overlap of quantified proteins across four replicates in HSCs or MPPs. f, Correlation of quantified proteins among four replicates in HSCs or MPPs. g, Venn diagrams showing the overlap of quantified acetylated peptides among four replicates in HSCs or MPPs. h, Correlation of quantified acetylated peptides among four replicates in HSCs or MPPs.