Extended Data Fig. 3: A CRAC channel blocker (La3+) was used to inhibit the Ca2+ influx in cells co-transfected with N-ST1 and C-TD in the presence of SA.
From: Repurposing salicylic acid as a versatile inducer of proximity
a) Time-course of whole cell inward currents at -100 mV. Upon whole-cell break-in (t = 0 s), a substantial inward Ca2+ current was observed in an external solution containing 10 mM Ca2+ with SA. When the standard external Ringer’s solution was replaced with a 20 mM Ca2+ external solution, an even larger Ca2+ current was detected. These currents were effectively blocked by La3+ at 50 µM. b) Quantification of the current density at 20 mM Ca2+, and 50 µM La3+. n = 4 from three independent biological replicates (mean ± s.e.m.).
