Supplementary Figure 4: Cell surface expression dynamics of B and T cell markers.

(a-d) Mouse splenic B cells were purified from either WT or TLR9 KO mice. Cells were cultured in vitro in the presence or absence of CpG (1 µM) and/or Anti-IgM (5 µg/ml). Cell surface expression levels of CD86 (a), CD80 (b), MHC-II (c) and CD69 (d) at 24 and 48h post stimulation are shown as histogram overlays (left) and MFI graphs (right). Data represents more than three independent experiments, each performed with triplicates. (e-h) Time course of the change in the Fold MFI for CD86 (e), MHC-II (f) and CD69 (g). Data are representative of two independent experiments performed in triplicates. (h) MFI graph for T cell CD44 expression for the experiment outlined in Fig. 5d. Data represents three independent experiements each carried out in triplicates. Statistical significance was measured using two sided unpaired t-test (n.s.= P>0.05; *=0.01<P≤0.05; **= 0.001< P ≤0.01).