Supplementary Figure 3: DPP4 does not truncate mouse CCL24 but reduces CCL11-mediated internalization of CCR3 in eosinophils.

(a) Recombinant mCCL24 was incubated in the presence or absence of mDPP4 and analyzed by mass spectrometry. Numbers indicate the molecular weight (in Daltons). (b) WT mice fed with Ctrl or with DPP4i were injected intravenously with 1 μg of CCL11 and blood was collected 1 h after injection. Representative histogram of CCR3 expression on eosinophils is shown. Quantification was determined by flow cytometry. Each dot represents one mouse (n = 7 (Ctrl) or 8 (DPP4i) mice). Data are representative of two independent experiments yielding similar results. Bars represent median values. NS, not significant; **P < 0.01. Significance was determined using or two-sided Mann–Whitney test.