Supplementary Figure 6: JUNB in PU.1-deleted neutrophils.
From: Safeguard function of PU.1 shapes the inflammatory epigenome of neutrophils
(a) Homer motif analysis in PU.1-bound cluster I and II or III and IV enhancers. Data were derived from two independent samples each. Motif enrichment was calculated with HOMER software, applying cumulative hypergeometric distribution adjusted for multiple testing with the Benjamini-Hochberg method. (b) Average and normalized (events per million) cutting events based on the ATAC-seq data over cluster enhancers containing a CEBP motif. (c) Genomic distribution of JUNB and PU.1 cobound regions in HoxER-PU.1WT neutrophils. (d) qPCR analysis of Junb expression in C. albicans stimulated HoxER-PU.1ΔNeu neutrophils that were infected with retroviruses carrying a scramble control or one of three independent shRNAs against Junb. Expression is shown as percent of the control value. Dashed line represents the mean expression value of the controls.
