Supplementary Figure 1: Functional screening of microRNAs in the anti−intracellular bacteria responses. | Nature Immunology

Supplementary Figure 1: Functional screening of microRNAs in the anti−intracellular bacteria responses.

From: Inducible degradation of lncRNA Sros1 promotes IFN-γ-mediated activation of innate immune responses by stabilizing Stat1 mRNA

Supplementary Figure 1

a, Listeria monocytogenes (L.m.) −induced cell death of PMs transfected with antago−NC (antg−NC) or antago−miR−1 (antg−miR−1). The survival of cells was determined 21 h later by 7−AAD uptake. b, qRT−PCR analysis of miR−1 mRNA in sorted mouse cells; results were normalized to U6. HSC, hematopoietic stem cell, NK cell, natural killer cell; macrophage. c, qRT−PCR analysis of mature miR−1 in peritoneal macrophages after intraperitoneal infection with L.m. for the indicated hours. Data were normalized to U6 expression levels. d,h, IFN−γ−induced gene expression level of Stat1 pre−mRNA after either treating with an antg−miR−1 (d) or knockout miR−1(h). e, Immunoblot analysis and quantification analysis of p−STAT1 and STAT1 and β−Actin in control or miR−1−overexpressed PMs after stimulation with IFN−γ for the indicated times. f,g, Expression levels of mature (f) or pre−mRNA transcript (g) of Stat1 in IFN−γ stimulated PMs after prior transfection with miR−1 mimics. Data are representative of three independent experiments (e) or three independent experiments with n = 3 biological replicates (a-h; shown as mean and s.e.m.). Individual data points represent individual biological replicates. *P<0.05, **P<0.01, ***P<0.001, two-tailed unpaired Student’s t−test.

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