Extended Data Fig. 7: WNT_inhibition_blocks_cytokine_induced_NK_cell_memory.
a, PBMCs were treated with or without LGK974 for 16 hrs. Percentage of Lin–TBX21+ cells, and the CD94– and CD94+ cells among the Lin–TBX21+ population, are indicated (left); data are representative of 4 anonymous HIV-1– blood donors. PBMCs were stimulated with IL-12 and IL-15 for 16 hrs in the absence or presence of LGK974. Live cells and Lin–CD56+ cells were examined (right); data are representative of 10 anonymous HIV-1– blood donors. b, NK cells without primary stimulation as in Fig. 7a were stimulated with IL-12 + IL-15, then IFN-γ production of cells with or without LGK974 was detected (n = 8), samples are from HIV-1– donors. c, Magnetic beads enriched NK cells from HIV-1– donors were transduced with lentivectors expressing GFP and shRNAs targeting either TCF7 or control, the TCF7 level in GFP+ cells was detected by flow cytometry. d, Control or TCF7 knockdown NK cell in c were treated as in Fig. 7a, percent IFN-γ+ among GFP+ cell were detected after secondary stimulation (n = 3). e, Correlation of ILCs with TCF7+NK cells. Samples are from HIV-1+ viremic individuals, ART suppressed HIV-1+ individuals (ART), and HIV-1+ individuals who spontaneously control viremia without ART. Cohort characteristics are described in Supplementary Table 1 (n = 53). The correlation coefficient (R) was determined by Pearson, p value for the slope being zero was determined by the F-test. Data are mean ± s.e.m; b,d, two tailed paired t-test, ns, not significant, *p < 0.01.
