Extended Data Fig. 4: CD8 T cells show metabolic phenocopies of monocytes and MDSCs after co-culture.
From: Regulatory myeloid cells paralyze T cells through cell–cell transfer of the metabolite methylglyoxal
a-f, analysis of anti-CD3/CD28 activated human CD8 T cells after coculture with MDSCs or monocytes (ratio 1:1) for 30 minutes. a, cell surface Glut-1 expression levels (n = 3 biological independent samples). b, glucose (2-NBDG) uptake (n = 3 biological independent samples). c, hexokinase activity from FACSorted CD8 T cells (triplicates; n = 3). d, glycolytic rates in FACSorted CD8 T cells by extracellular flux analysis (n = 3 biological independent samples). e, oxygen consumption rates (OCR) of FACSorted CD8 T cells (n = 3 biological independent samples). f, ATP content of CD8 T cells after FACSorting (n = 3 biological independent samples;). ns = not significant; *p < 0.05; **p < 0.01; ***p < 0.001; two-way unpaired t-test plotted as SEM.
