Extended Data Fig. 6: Single cell RNA-seq analysis of neutrophils in E. coli-challenged mice.
From: Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection
a, Number of white blood cells and the proportion of neutrophils in mice before and after E. coli challenge evaluated by a hematology analyzer (Mindray BC-5000 Vet). Results are the mean ± SD of three independent experiments. b, Experimental scheme of the sample collection process after E. coli challenge. c, Summary of sample information. Organ distribution of neutrophils is shown on the right. d, Cell viability percentages immediately before cells were loaded into the 10X Chromium Controller. e, UMAPs of all 24,943 cells from BM, PB, and SP from E. coli-challenged mice colored by sample origin and cell type, respectively. f, Heatmap showing row-scaled expression of the five highest DEGs for all QC-passed cells colored by cell type. g, Comparisons of the number of genes, number of UMIs, mitochondria count percentage, and UMI per gene of all QC-passed cells in each organ before and after E. coli challenge. h, As in g but only of all neutrophils in each organ. i-j, Heatmaps showing expression of 7 genes of the NADPH oxidase complex (i) and neutrophil granule-related genes (j) for all neutrophils.
