Extended Data Fig. 9: Single-cell RNA-seq analysis of human peripheral blood neutrophils.
From: Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection
a, Overview of study design and the gating strategy for isolating human PB neutrophils. b, UMAP plots of neutrophils from three healthy donors (D1, D2, or D3) colored by cluster identity. c, The combined UMAP plot of the three donors. d, Dot plot showing scaled expression of selected signature genes for each cluster colored by average expression of each gene in each cluster scaled across all clusters. Dot size represents the percentage of cells in each cluster with more than one read of the corresponding gene. The analysis was conducted using cells from all three human donors. e, Row-scaled expression of the ten highest differentially expressed genes (Bonferroni-corrected P values < 0.05, Student’s t-test) in each neutrophil cluster. D1+D2+D3, the analysis was conducted using cells from all three human donors. f, Row-scaled expression of 37 interferon-stimulated genes in each neutrophil cluster. The analysis was conducted using cells from all three human donors.
