Fig. 2: Detection of SARS-CoV-2 nonstructural protein antibodies by LIPS and sums.
From: ORF8 and ORF3b antibodies are accurate serological markers of early and late SARS-CoV-2 infection
a–h, Antibodies against NSP1 (a) (in ORF1ab) and other ORFs (ORF3a (b), ORF3b (c), ORF6 (d), ORF7a (e), ORF7b (f), ORF8 (g) and ORF10 (h)) were measured by LIPS to cover all the ORFs of the virus. i–l, Cumulative antibody LIPS levels to the SARS-CoV-2 antigens in patients with COVID-19 and healthy controls for 11 relevant antigens (N, M, S, S1, S2′, NSP1, ORF3a, ORF3b, ORF7a, ORF7b and ORF10) (i), three most sensitive antigens (N, ORF3b and ORF8) (j), selective antigens (ORF3b and ORF8) (k) and three S-relevant antigens (S, S1 and S2′) (l). The cutoff value is shown by the dotted line and was based on the mean + (3 × s.d.) of the negative control group. Blue dots represents data points of patients with COVID-19 before day 14. Experiments were repeated twice. Two-sided P values were calculated using the Mann–Whitney U-test. Asterisks denote statistical significance between patients with COVID-19 versus negative controls. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001. All data represent the mean ± s.d. and individual responses (n = 15); background values were subtracted.
