Extended Data Fig. 6: INKA1-OE mediated effects in in vitro differentiation assays.
From: A latent subset of human hematopoietic stem cells resists regenerative stress to preserve stemness
a, CB LT- and ST-HSC were cultured, transduced (OE) and on day 3 ptd BFP+ cells were sorted into methylcellulose for colony formation, scored and harvested 12 days later and replated (right) for 12 additional days (n = 4). Colony type composition of primary and secondary colony formation assays (CFU-C) is shown in the lower panels; Mean ± SD, two-sided paired t-test. b, Total single cell colony output (d15) from stromal coculture assays per CB (n = 3 independent pools, Mean ± SD) and subfraction of CTRL and INKA1-OE transduced HSC subsets as indicated. My = myeloid (CD33+), E = erythroid (CD45-GlyA+/CD71+), Mk = megakaryocytic (CD41+), NK = CD56+cells. Upper right panel shows proliferative potential and lower panel total colony output per assay, population and condition as assessed by flow cytometry; two-sided paired t-test.
