Extended Data Fig. 4: ILC2-dependent anti-tumor immunity in ICOS-T mice.

Ret tumor growth and tumor immune cell composition of C57BL/6 J and ICOS-T mice (CD4^Cre/+ICOS^fl-DTr/+) treated with PBS or rmIL-33 and/or diphtheria toxin (DTx). a, Experimental design and treatment regime for amplification and deletion of ILC2 in vivo. b, Ret tumor growth (left panel) and tumor weight (g) (right panel) at day 17. Data show the mean ± s.e.m. Each dot represents one mouse. Statistical differences were assessed using an unpaired Student’s t test between the groups ICOS-T + IL-33 and ICOS-T DTx + IL-33 and the p value is indicated. Data show C57BL/6 J + PBS (tumor growth, n = 3; tumor weight, n = 2 mice), C57BL/6 J + IL-33 (tumor growth, n = 3; tumor weight, n = 3 mice), ICOS-T + IL-33 (tumor growth, n = 6; tumor weight, n = 4 mice), ICOS-T + IL-33 + Dtx (tumor growth, n = 6; tumor weight, n = 4 mice). c,d, Representative flow cytometric contour plots (left panels) and quantification (middle panels) of tumor-infiltrating ILC2 (CD45⁺lin^-(CD3ε^-TCRβ^-CD11b^-NK1.1^-NKp46^-) RORγt^-GATA3⁺) and regulatory CD4⁺ T cells (Tregs, CD45⁺CD3ε⁺TCRβ⁺CD8^-CD4⁺CD25⁺Foxp3⁺) in each treatment. Data show mean ± s.e.m. (middle panels). Dot plots show the correlation of the frequency of (c, right) tumor infiltrating ILC2 or (d, right) Tregs with tumor weight (g) (right panels). Individual data points are colored by experimental group. Correlations were assessed using non-parametric Spearman’s correlation tests and overlaid by linear regression curves. Each dot represents one mouse (n = 13 mice). Spearman’s Rho (r_s) and p-values are indicated. e, Frequency of tumor-infiltrating CD8⁺ (live CD45⁺CD3ε⁺TCRβ⁺CD8⁺CD4^-) T cells. Data show mean ± s.e.m (left panel). Dot plots showing correlation between the frequency of tumor-infiltrating CD8⁺ T cells and tumor weight (g) (right panel). Individual data points are colored by experimental group. The correlation was assessed using a non-parametric Spearman’s correlation test. A simple linear regression curve was been overlaid on data for individual animals (n = 13) from one experiment. Spearman’s Rho and p-values are indicated.