Extended Data Fig. 1: Flow cytometric analyses of innate lymphoid cell subsets in BRAFCA;PTENloxp;Tyr::CreERT2 mice.
From: Blockade of the co-inhibitory molecule PD-1 unleashes ILC2-dependent antitumor immunity in melanoma
a, Kinetics of individual tumor growth in BRAFCA;PTENloxp;Tyr::CreERT2 mice. Tumor induction was performed at day 0. Individual data were pooled from 3 independent experiments (n = 16, 3–9 mice per experiment). Each line shows the growth curve for an individual mouse and the mean growth is shown in dark red. HT, hydroxytamoxifen. b, Representative flow cytometric contour plots showing the gating strategy used to identify tumor-infiltrating immune cell populations. c, Frequency of NK cells, ILC1, ILC2 and ILC3 within skin and tumors. The skin was collected from flank on the opposite side to tumor induction. Tumor and skin-resident immune cell populations were identified as indicated in b. Data are pooled from 4 independent experiments (n = 14 mice; 2–5 mice/experiment). c, Each dot represents one mouse and data show mean ± s.e.m. Statistical analyses were performed using a Student’s paired t test. p-values are indicated.
