Extended Data Fig. 5: Detection of the components of sphingolipin in NK cells by sIC-MS and bulk LC-MS.
From: Tumors evade immune cytotoxicity by altering the surface topology of NK cells
a, sIC-MS and bulk LC-MS in detecting standard of sphingomyelin (SM(d34:1) and SM(d36:1)). The range of concentration from 0.2 to 50 mg/L. With sIC-MS, for the standard curve of SM(d34:1), the slope was 2.021 × 10−2, R2 = 0.9925 and the standard curve of SM(d36:1)), the slope was 2.012 × 10−2, R2 = 0.9977. With bulk LC-MS, for the standard curve of SM(d34:1), the slope was 1.977 × 10−2, R2 = 0.9981. And the standard curve of SM(d36:1)), the slope was 1.979 × 10−2, R2 = 0.9923. b, LC-MS was used to analyse the SM content of purified peripheral NK cells from healthy donors. Freshly purified NK cells were treated with D609 (25, 100, 400 μM) or PBS (control) for 24 h. n = 5 samples per group. Data are the mean ± s.d. Data were analysed by two-way ANOVA.
