Extended Data Fig. 7: The potential relationships between cholesterol and sphingomyelin levels in NK cells.
From: Tumors evade immune cytotoxicity by altering the surface topology of NK cells
a, CLSM showing the cholesterol signals for purified intratumoral NK cells, liver NK cells and peripheral NK cells by filipin III staining. Scale bar, 1 μm. b–c, CLSM of filipin III stained purified peripheral NK cells staining (cyclodexrin treated or untreated (vehicle, PBS); D609 treated or untreated (vehicle, PBS) to assess cholesterol levels. sIC-MS was used to analyse the membrane SM content of purified peripheral NK cells (cyclodexrin treated or PBS). Scale bar, 1 μm. n = 9 in each group. d, SEM image showing the membrane protrusions of purified peripheral NK cells. Peripheral NK cells were treated with cyclodextrin. Each dot represents one sample. n = 5 samples per group. Scale bar, 1 μm. Data are the mean ± s.d. Data were analyzed by two-tailed unpaired Student’s t-test. e, Purified peripheral NK cells were co-cultured with HepG2 cells for cytotoxicity assays after treatment of cyclodextrin. n = 4 samples per group. f, Single-cell MS was employed to analyze the cholesterol in intratumoral NK cells, peripheral NK cells, and liver NK cells. n = 5 samples per group. Data are the mean ± s.d. Data were analyzed by two-tailed unpaired Student’s t-test.
