Extended Data Fig. 1: Imaging of membrane protrusions of NK cells.
From: Tumors evade immune cytotoxicity by altering the surface topology of NK cells
a, Gating strategy of NK cells for flow cytometry. b, Representative density plots show the purity of NK cells. c–d, Related to Fig. 1a–g, The mean diameter of membrane protrusions (c, TEM; d, SEM) in each sample is shown. Each dot represents one sample. n = 10 in each group. e–g, CLSM shows the membrane protrusions of purified NK cells. e, Representative 2D CLSM images (up) and 3D resultant ‘cartoon’ images (down). Scale bar, 1 μm. f–g, The mean number of membrane protrusions and mean length of membrane protrusions (2D CLSM) in each sample are shown. Each dot represents one sample. n = 8 in each group. Data are the mean ± s.d. Data were analysed by two-way ANOVA.
